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采用浓集G_1相细胞的淘式离心方法分离小鼠乳癌细胞66,即从培养第2天的P细胞中分得P-G_1部分,由培养第7天的Q细胞中分得Q-G_1(G_0/G_1)部分。淘式离心后的P-G_1和Q-G_1细胞,其体积相应比分离前的P和Q减小,通过FCM检检测,分离后两亚群中90％以上的细胞属G_1相;但连续H~3标记结果显示,P-G_1的标记指数和P细胞接近高达97.1％,Q-G_1的标记指数比Q细胞低仅1.7％;放射存活曲线表明,分离前的Q细胞比细胞放射敏感性高,分离后P-G_1的放射反应基本与P细胞相同,而Q-G_1的放射敏感性高于Q细胞。实验证明,在具有同样DNA含量的G_1相细胞,由于原来所处的生长状态不同(P-G_1来自P细胞,Q-G_1来自Q细胞),共细胞体积、增殖能力和放射敏感性均现出其固有的异质特性。
The mouse breast cancer cells 66 were isolated by means of the centrifugation method of collecting concentrated G_1 phase cells, that is, the P-G_1 fraction was obtained from the P cells on the second day of culture, and the Q-G_1 fraction was obtained from the Q cells on the seventh day G_0 / G_1) section. After centrifugation, the P-G_1 and Q-G_1 cells had a correspondingly smaller volume than the P and Q before the separation. More than 90% of the cells in the two subpopulations were G_1 phase after FCM assay. However, continuous H The labeling results of ~ 3 showed that the labeling index and P cells of P-G 1 were as high as 97.1% and the labeling index of Q-G 1 was only 1.7% lower than that of Q cells. The radiation survival curve showed that the Q cells before isolation were higher radiosensitivity than the cells , The radioactivity of P-G 1 after isolation was almost the same as that of P cells, while the radiosensitivity of Q-G 1 was higher than that of Q cells. Experiments show that in the G_1 phase cells with the same DNA content, the co-cell volume, proliferative capacity and radiosensitivity are all shown due to the different growth states (P-G_1 from P cells and Q-G_1 from Q cells) Its inherent heterogeneity.