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目的研究慢性阻塞性肺疾病(简称慢阻肺)大鼠模型中白细胞介素10(IL-10)、肿瘤坏死因子ɑ(TNF-α)、干扰素-γ(IFN-γ)在血清和肺组织中的表达,探讨IL-10在慢阻肺气道炎症中的作用及调控机制。方法成年雄性SD大鼠45只,分为模型组30只,对照组15只,采用反复熏香烟+气管内注入脂多糖法建立慢阻肺大鼠模型。ELISA法检测大鼠血清、肺组织中IL-10、TNF-ɑ和IFN-γ的表达。结果模型组血清和肺组织内TNF-α较对照组显著升高,IFN-γ、IL-10[血清(44.68±8.67)ng/L比(75.96±10.59)ng/L,肺组织(64.55±9.03)ng/L比(94.06±8.71)ng/L,P均<0.01]表达均下降。IL-10和TNF-ɑ水平呈负相关(血清r=-0.67,肺组织r=-0.80,P<0.01)。IL-10和IFN-γ水平呈正相关(血清r=0.64,肺组织r=0.72,P<0.01)。结论 IL-10的下调在慢阻肺大鼠气道炎症中起重要的调控作用。
Objective To investigate the expression of interleukin 10 (IL-10), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in serum and lung of chronic obstructive pulmonary disease (COPD) To explore the role of IL-10 in chronic obstructive pulmonary disease (COPD) and its regulatory mechanism. Methods Forty-five adult male Sprague-Dawley rats were divided into model group (n = 30) and control group (n = 15). Chronic obstructive pulmonary disease model was established by repeated cigarette smoking and intratracheal instillation of lipopolysaccharide. The levels of IL-10, TNF-ɑ and IFN-γ in serum and lung tissue were detected by ELISA. Results The levels of TNF-α in serum and lung tissue in model group were significantly higher than those in control group. The levels of IFN-γ, IL-10 [serum (44.68 ± 8.67) ng / L vs 75.96 ± 10.59 9.03) ng / L (94.06 ± 8.71) ng / L, all P <0.01]. IL-10 and TNF-ɑ levels were negatively correlated (serum r = -0.67, lung tissue r = -0.80, P <0.01). IL-10 and IFN-γ levels were positively correlated (serum r = 0.64, lung tissue r = 0.72, P <0.01). Conclusion The down-regulation of IL-10 plays an important role in airway inflammation in COPD rats.