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目的探讨TNF-α、IL-6和IL-8的变化及其在哮喘发病中的意义。方法采用ELISA法检测120例哮喘病急性加重期及治疗后稳定期和80例健康对照者外周血TNF-α、IL-6和IL-8水平,同时测定血清中总IgE水平和第1秒用力呼气容积占预计值百分比(FEV1%)。结果哮喘急性加重期组外周血TNF-α、IL-6和IL-8和IgE水平明显高于治疗后稳定期组和对照组(t=-20.79～17.99,P<0.01)。治疗后稳定期组TNF-α、IL-6和IL-8和IgE亦显著高于对照组(t=-16.21～16.10,P<0.01)。哮喘急性发作期与治疗后稳定期外周血TNF-α、IL-6和IL-8与FEV1%呈负相关(r分别为-0.693、-0.728、-0.533,均P<0.01)。结论 TNF-α、IL-6和IL-8参与了哮喘气道炎症的形成,并与气道阻塞程度密切相关。
Objective To investigate the changes of TNF-α, IL-6 and IL-8 and their significance in the pathogenesis of asthma. Methods The levels of TNF-α, IL-6 and IL-8 in peripheral blood of 120 asthma exacerbation and post-treatment stable and 80 healthy controls were measured by ELISA. Total serum IgE level and the first second force Exhalation volume percentage of expected value (FEV1%). Results The levels of TNF-α, IL-6, IL-8 and IgE in the peripheral blood of asthma exacerbation group were significantly higher than those in the stable group and the control group (t = -20.79-17.99, P <0.01). The levels of TNF-α, IL-6, IL-8 and IgE in stable group after treatment were also significantly higher than those in control group (t = -16.21 ~ 16.10, P <0.01). The levels of TNF-α, IL-6, IL-8 and FEV1% in the asthma exacerbation and post-treatment stable phases were negatively correlated (r = -0.693, -0.728, -0.533, all P <0.01). Conclusion TNF-α, IL-6 and IL-8 are involved in the formation of airway inflammation in asthma and are closely related to the degree of airway obstruction.