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目的建立一种稳定的大鼠脑局灶性梗死模型,以用于脑梗死后神经干细胞移植的长期观察。方法37只大鼠随机分为实验组和对照组。微创法经颞骨局部钻孔开颅,采用直接结扎大脑中动脉终段,同时永久结扎同侧颈总动脉、暂时性夹闭对侧颈总动脉的方法制备大鼠脑梗死模型。通过大鼠脑梗死后的神经功能评分、墨汁灌注、TTC染色、MRI成像结果对该模型进行评价。结果大鼠术后状态良好,实验组大鼠观察4周后死亡率低为6 .25 %。大鼠神经功能评分均为1分,墨汁灌注及TTC染色观察梗死范围局限于皮层,4周后MRI成像测量梗死体积稳定,平均为83 .52 mm3。结论该模型对大鼠创伤小,梗死灶的位置和体积恒定,长期存活率高,为脑梗死后神经干细胞移植的研究提供了一种理想的动物模型。
Objective To establish a stable model of focal cerebral infarction in rats for long-term observation of neural stem cell transplantation after cerebral infarction. Methods 37 rats were randomly divided into experimental group and control group. Minimally invasive craniotomy was performed by local drilling of the temporal bone. The cerebral infarction model was prepared by ligating the terminal segment of the middle cerebral artery directly and permanently ligating the common carotid artery for a temporary occlusion of the common carotid artery. The model was evaluated by neurological function score, ink perfusion, TTC staining and MRI imaging after cerebral infarction in rats. Results The rats were in good condition after operation. The mortality of rats in experimental group was 6.25% after 4 weeks observation. Nerve function scores of rats were 1 point. Infarct perfusion and TTC staining showed that infarct size was confined to the cortex. Infarct size was stable after MRI imaging for 4 weeks with an average of 83.52 mm 3. Conclusion The model of traumatic injury in rats, infarction location and constant volume, long-term survival rate, for the study of neural stem cell transplantation after cerebral infarction provides an ideal animal model.