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目的:探讨丝裂霉素C联合蛙皮素在体外诱导人胃癌细胞的凋亡作用。方法:利用体外细胞培养、MTT比色技术及流式细胞仪等方法,在对照组、丝裂霉素C(MMC)组、蛙皮素(BOM)组和MMC+BOM组中,用不同浓度的BOM及MMC,对人胃癌细胞株SGC7901的生长曲线、细胞抑制率和细胞凋亡率的影响。结果:在一定浓度范围内,BOM对胃癌细胞的促生长作用及MMC的抑制作用均呈时间-剂量依赖性。BOM组、对照组、MMC组和MMC+BOM组的胃癌细胞生存率依次为90.00%、82.96%、65.07%、60.14%;凋亡率在MMC+BOM组明显增高(29.26%),与对照组(4.72%)或BOM组(5.13%)两两比较时,具有显著性差异(P<0.01);MMC+BOM组凋亡率(29.26%)较M M C组(25.11%)高,在统计学上具有差异性意义(P<0.05)。结论:虽然BOM在体内外能刺激胃癌细胞增殖,但与抗癌药MMC联合后,可协同MMC对胃癌细胞的凋亡,提高抗癌药的疗效。
Objective: To investigate the apoptosis of human gastric cancer cells induced by mitomycin C combined with bombesin in vitro. METHODS: Using in vitro cell culture, MTT colorimetric techniques, and flow cytometry, different concentrations were used in the control group, the mitomycin C (MMC) group, the bombesin (BOM) group, and the MMC+BOM group. The effects of BOM and MMC on the growth curve, cell inhibition rate and apoptosis rate of human gastric cancer cell line SGC7901. Results: In a certain range of concentration, the growth-promoting effect of BOM on gastric cancer cells and the inhibitory effect of MMC were time-and dose-dependent. The survival rates of gastric cancer cells in the BOM group, control group, MMC group, and MMC+BOM group were 90.00%, 82.96%, 65.07%, 60.14%, respectively; the apoptosis rate was significantly higher in the MMC+BOM group (29.26%), compared with the control group. (4.72%) or BOM group (5.13%) showed a significant difference (P<0.01); MMC+BOM group had a higher apoptotic rate (29.26%) than MMC group (25.11%), statistically There is a difference in significance (P<0.05). Conclusion: Although BOM can stimulate the proliferation of gastric cancer cells in vivo and in vitro, combined with anti-cancer drug MMC, it can synergize the apoptosis of gastric cancer cells with MMC and improve the efficacy of anti-cancer drugs.