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目的检测MMP-3、MMP-10和基质金属蛋白酶抑制剂2(TIMP-2)在结肠癌组织中的表达。方法采用免疫组织化学方法检测MMP-3、MMP-10及TIMP-2在60例结肠癌和26例癌旁正常组织中的表达。结果结肠癌组织中MMP-3、MMP-10和TIMP-2阳性表达率分别为76.7%、58.3%和66.7%,高于癌旁正常组织的15.4%、11.5%和3.84%(P<0.01)。MMP-3和MMP-10的表达均与肿瘤大小、浸润深度、淋巴结转移及TNM分期密切相关(P<0.05或P<0.01);TIMP-2表达与淋巴结转移密切相关(P<0.05)。MMP-3、MMP-10表达与TIMP-2表达有相关性(r=0.33、r=0.28,P<0.05)。结论 MMP-3、MMP-10和TIMP-2的高表达与结肠癌侵袭与转移密切相关,可以作为临床评估结肠癌进展及肿瘤预后的指标。
Objective To detect the expression of MMP-3, MMP-10 and TIMP-2 in colon cancer tissues. Methods Immunohistochemistry was used to detect the expression of MMP-3, MMP-10 and TIMP-2 in 60 cases of colorectal cancer and 26 cases of adjacent normal tissues. Results The positive rates of MMP-3, MMP-10 and TIMP-2 in colon cancer tissues were 76.7%, 58.3% and 66.7%, respectively, which were higher than those in adjacent normal tissues (15.4%, 11.5% and 3.84%, P <0.01) . The expression of MMP-3 and MMP-10 were closely related to tumor size, depth of invasion, lymph node metastasis and TNM stage (P <0.05 or P <0.01). TIMP-2 expression was closely related to lymph node metastasis (P <0.05). The expression of MMP-3 and MMP-10 was correlated with the expression of TIMP-2 (r = 0.33, r = 0.28, P <0.05). Conclusion The high expressions of MMP-3, MMP-10 and TIMP-2 are closely related to the invasion and metastasis of colon cancer, which may be used as an index to evaluate the progress of colon cancer and the prognosis of cancer.