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为了解健康儿童空腹时血清中游离氨基酸含最的正常值,以及与性成熟的关系,作者共测定了64名健康男童与57名健康女童,并按性别分成5～10岁和10～15岁两个年龄组。为避免饮食的影响,只测定营养正常儿童的空腹血清,因为一次蛋白质丰富的进餐,可使血浆中的氨基酸升高50～100%。取血后立即分离血清,以1:1(V/V)5%磺基水杨酸去除蛋白质后将血清标本贮存于-20℃冰箱内,供分析用。游离氨
In order to find out the most normal values of serum free amino acids in healthy children and their relationship with sexual maturity, 64 healthy boys and 57 healthy girls were tested and divided into 5 to 10 years old and 10 to 15 Two age groups. In order to avoid the effects of diet, only the fasting serum of children with normal nutrition is measured. As a protein-rich meal, the amino acids in plasma can be increased by 50-100%. Serum samples were taken immediately after the blood was taken and the serum samples were stored in a -20 ° C freezer at 1: 1 (V / V) 5% sulfosalicylic acid for analysis. Free ammonia