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目的观察树（Ｔｕｐａｌａ）经人工传代感染ＨＤＶ后肝内ＨＤＡｇ表达及病理变化方法驯养的野生Ｔｕｐａｉａ第一代接种人ＨＤＶ阳性血清，第二、三代分别接种第一、二代感染ＨＤＶ成功的Ｔｕｐｓｉａ血清０，４ｍＬ／只，感染前３ｗｋ所有Ｔｕｐａｉａ均接种了人ＨＢＶ阳性血清０．４ｍＬ／只，接种ＨＤＶ前及以后每周取血及肝脏标本一次，连续６ｗｋ肝组织石蜡包埋，分别采用ＨＥ及ＳＰ法染色观察病理变化及抗原表达，原位杂交法观察ＨＤＶＲＮＡ．结果第一代１／１只，第二代３／４只，第三代５／５／ｑＴｕｐａｉａ于感染ＨＤＶ阳性血清２ｗｋ后肝内均可检出ＨＤＡｇ，持续至ｗｋ６观察结果时，ＨＤＡｇ定位于肝细胞核和浆内，少数呈膜型，阳性肝细胞呈片状分布，亦可散在表达于单个肝细胞内，ＨＤＶＲＮＡ主要在肝细胞核内表达肝组织呈病毒性肝炎样病理变化，如变性点灶状坏死及汇管区炎细胞浸润．结论Ｔｕｐｓｉａ传代感染ＨＤＶ肝脏病理变化及ＨＤＡｇ表达与在黑猩猩中十分类似，提示Ｔｕｐａｔａ可作为感染ＨＤＶ实验动物
Objective To observe the HDV-positive sera of the first generation vaccinated human Tupaia domesticated by the way of HDAg expression and pathological changes in Tupala infected by HDV. The second and third generation were respectively infected with the first and second generation HDV-infected Tupsia serum 0,4mL / only, 3wk before infection all Tupaia were inoculated with human HBV-positive serum 0.4mL /, before and after inoculated with HDV blood and liver samples once a week, continuous 6wk liver tissue paraffin embedding, respectively, using HE and Pathological changes and antigen expression were observed by SP staining and HDV RNA was observed by in situ hybridization. Results The first generation of 1/1, the second generation of 3/4, the third generation of 5/5 / qTupaia in HDV positive serum 2wk after detection of HDAg in the liver, until the wk6 observations, HDAg located in Hepatic nuclei and plasma, a small number of membranous, positive hepatocytes were flaky distribution, but also scattered in a single hepatocyte expression, HDVRNA mainly in the liver nucleus of hepatic tissue showed viral hepatitis-like pathological changes, such as degeneration Necrosis and inflammatory cells in the portal area infiltration. Conclusion The pathological changes of HDV liver and the expression of HDAg in the Tupsia-infected HDV liver are similar to those in chimpanzees, suggesting that Tupata may be used as an experimental animal for HDV infection