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目的:研究灵芝孢子对帕金森病(PD)大鼠模型黑质神经细胞caspase-3的影响。方法:SD大鼠随机分为3组, PD组:经立体定向向黑质部注入6-羟多巴(6-OHDA);灵芝孢子组:先用灵芝孢子粉灌胃3d,立体定向注入6-OHDA,继续灌胃4周;正常对照组:立体定向注入抗坏血酸生理盐水。实验大鼠4周处死后用免疫组化、原位杂交检测caspase-3及其mRNA的阳性细胞数,Western blot检测caspase-3的半定量。结果:灵芝孢子组术侧黑质caspase-3及其mRNA阳性神经元数量较PD组明显降低,Western blot显示caspase-3蛋白较PD组显著降低。结论:灵芝孢子能够降低caspase-3的表达,对PD大鼠有脑保护作用。
Objective: To study the effect of Ganoderma lucidum spores on caspase-3 in substantia nigra neural cells of rat model of Parkinson’s disease (PD). METHODS: SD rats were randomly divided into 3 groups: PD group: 6-hydroxydopamine (6-OHDA) was injected into the substantia nigra by stereotaxy; Ganoderma lucidum spore group: first gavage with Ganoderma lucidum spore powder for 3 days, stereotactic injection 6 -OHDA was continued for 4 weeks; normal control group: stereotaxic injection of ascorbic acid saline. After the rats were sacrificed at 4 weeks, the number of positive cells of caspase-3 and its mRNA was detected by immunohistochemistry and in situ hybridization, and semi-quantitation of caspase-3 was detected by Western blot. Results: Compared with PD group, the number of nigral caspase-3 and its mRNA positive neurons in the ganoderma lucidum spore group was significantly lower than that in the PD group. Western blot showed that the caspase-3 protein was significantly lower than that in the PD group. CONCLUSION: Ganoderma lucidum spores can reduce the expression of caspase-3 and have a protective effect on PD rats.