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目的探讨三七总皂苷(TSPN)对全脑缺血后成年大鼠海马胶质纤维酸性蛋白(GFAP)表达及脑水肿的影响。方法采用四血管阻断法制作全脑缺血模型,大鼠被随机分成假手术组、模型组和TSPN组。TSPN组大鼠全脑缺血后30 min ip 75 mg/kg TSPN,每天1次,假手术组和模型组ip给予等体积的生理盐水,连续14 d。分别于再灌注1、3、7、14 d后处死。采用干湿法计算各组大鼠脑组织含水量,免疫组织化学染色观察海马齿状回(SGZ)区GFAP的表达,免疫荧光双标观察SGZ区GFAP/DCX的共表达情况,免疫印迹技术检测海马CA1区GFAP蛋白表达水平。结果 TSPN组大鼠脑组织含水量显著低于模型组(P<0.05);TSPN组、模型组SGZ区GFAP+细胞密度值在3、7、14 d有统计学差异(P<0.05);两组SGZ区GFAP/DCX共表达细胞与DCX阳性表达细胞数目的比值在第7、14天差异显著(P<0.05);免疫印迹结果显示TSPN组海马CA1区GFAP蛋白表达水平在第3、7、14天显著高于模型组(P<0.05)。结论 TSPN可通过促进全脑缺血后大鼠SGZ区星形胶质细胞活化、促进神经再生、减轻脑水肿而发挥神经保护作用。
Objective To investigate the effect of total saponins of Panax notoginseng (TSPN) on glial fibrillary acidic protein (GFAP) expression and brain edema in adult rats after global cerebral ischemia. Methods The model of global cerebral ischemia was established by four-vessel occlusion method. The rats were randomly divided into sham operation group, model group and TSPN group. Rats in TSPN group were given ip 75 mg / kg TSPN once a day for 30 min after ischemia. Rats in sham operation group and model group were given ip with equal volume of saline for 14 days. Were killed after 1,3,7,14 d reperfusion. The water content of brain tissue in each group was calculated by wet-dry method. The expression of GFAP in hippocampal dentate gyrus (SGZ) was observed by immunohistochemical staining. The co-expression of GFAP / DCX in SGZ was observed by immunofluorescence. The level of GFAP protein in hippocampal CA1 area. Results The water content of brain tissue in TSPN group was significantly lower than that in model group (P <0.05). The GFAP + cell density in SGZ of TSPN group and model group was significantly different at 3, 7 and 14 days (P <0.05) The ratio of GFAP / DCX co-expressing cells and DCX positive cells in SGZ area was significantly different at the 7th and the 14th day (P <0.05). Western blotting showed that the expression of GFAP protein in hippocampal CA1 area of TSPN group was significantly higher than that of DCX positive cells on day 3,7,14 Day was significantly higher than the model group (P <0.05). Conclusion TSPN can play a neuroprotective role in promoting glial cell activation, promoting nerve regeneration and relieving cerebral edema in rat SGZ after global cerebral ischemia.