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在实验室内用鲫鱼分别暴露于含有PCDD/Fs和PCBs严重污染的沉积物和未受污染的沉积物中,测定鱼肝脏中细胞色素P4501A1(CYP1A1)依赖的7-乙氧基-3-异吩唑酮-脱乙基酶(EROD)的活性。结果表明,鲫鱼暴露于受二类化合物污染沉积物中1周后,肝脏中EROD的活性是对照组的83倍,而未被污染的沉积物暴露试验中EROD的活性和对照组没有明显差别。在此沉积物-水实验系统中二类污染物的TCDD等当量浓度(TEQ/L)与肝脏EROD的活性有较好的相关性。较长时间暴露,得到肝脏EROD的活性随时间的变化关系。还获得了试验温度对EROD活性诱导的影响。此实验表明,用实验室鲫鱼暴露于沉积物-水系统中,鱼肝脏EROD活性诱导可作为沉积物毒性评价指标,用于受二类化合物污染的沉积物样品的筛选,并有希望成为此类污染物的半定量检测方法。
In the laboratory, the carp were exposed to heavily contaminated sediments and uncontaminated sediments containing PCDD / Fs and PCBs, respectively, and cytochrome P4501A1 (CYP1A1) -dependent 7-ethoxy-3- The activity of phenoxazolone-deethylase (EROD). The results showed that the activity of EROD in liver was 83-fold more than that of the control one week after the carp was exposed to the sediment contaminated by the dioxins, while the activity of EROD in the uncontaminated sediment exposure test was not significantly different from that in the control group difference. In this sediment-water experimental system, the equivalent TCDD concentrations (TEQ / L) of the two pollutants were correlated well with the liver EROD activity. After prolonged exposure, the changes of liver EROD activity were obtained over time. The effect of test temperature on EROD activity induction was also obtained. This experiment showed that exposure of laboratory crucian to the sediment-water system induced the EROD activity of fish liver as an indicator of sediment toxicity for the screening of sediment samples contaminated with dioxins and was promising to be Semi-quantitative detection of such contaminants.