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当人胚肺成纤维细胞被传至40代龄后,在培养液中加入人参根皂甙(SRG)sμg/ml,人参果皂甙(SFG)1.5μg/ml和人参茎叶皂甙(SSLG)1.0μg/ml可分别延长细胞传代寿命的120%,110%和130%。当培养液中三种皂甙的终浓度分别在1.78~16.00μg/ml,0.50~4.50μg/ml和0.25~4.00μg/ml范围内时。实验结果表明,高代龄细胞的饱和密度明显增加,细胞的克隆生长也受到显著影响,SRG的作用较SFG和SSLG更明显。而同样条件下三种皂甙对低代龄细胞的影响则不明显。
When human embryonic lung fibroblasts were passed to 40 generations of age, ginseng root saponin (SRG) sμg/ml, ginseng fruit saponin (SFG) 1.5 μg/ml, and ginseng stem saponin (SSLG) 1.0 μg/ml were added to the culture solution. The ml can prolong the cell passage life of 120%, 110% and 130%, respectively. When the final concentrations of the three saponins in the culture medium were 1.78 to 16.00 μg/ml, 0.50 to 4.50 μg/ml, and 0.25 to 4.00 μg/ml, respectively. The experimental results showed that the saturation density of high-generation cells increased significantly, and the clonal growth of cells was also significantly affected. The effect of SRG was more pronounced than that of SFG and SSLG. Under the same conditions, the effects of three saponins on low-age cells were not obvious.