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从湖南邵阳地区患出血病的草鱼组织中分离到一株病毒,经部分提纯、负染后,电镜观察,揭示为双层衣壳、直径约71nm的球形颗粒。其核酸在聚丙烯酰胺凝胶电泳中呈现11条带。经核酸酶消化、吖啶橙染色及核酸的变性试验,证明为双链RNA。病毒在草鱼肾细胞株(CIK)单层细胞上,形成直径约2mm的空斑;对温度有一定稳定性;对乙醚、氯仿不敏感;经pH3、10处理后仍能保持相对稳定的滴价。此病毒不属业已建立的呼肠孤病毒科6个属中的任何一个属,而可能为呼肠孤病毒科中一新成员。
A virus was isolated from grass carp tissues of hemorrhagic disease in Shaoyang of Hunan Province. After partially purified, negatively stained, and electron microscopically observed, it was revealed as a double-walled shell with spherical particles of about 71 nm in diameter. The nucleic acid showed eleven bands in polyacrylamide gel electrophoresis. After nuclease digestion, acridine orange staining and nucleic acid denaturation test, proved to be double-stranded RNA. Virus in the grass carp kidney cell line (CIK) monolayer cells, the formation of a diameter of about 2mm plaque; to temperature stability; insensitive to ether, chloroform; pH3,10 after treatment can still maintain a relatively stable price . This virus does not belong to any of the 6 genera established in the reovirus family and may be a new member of the family of reoviruses.