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目的:探讨Apelin基因rs2235306位点多态性与支气管哮喘(哮喘)和代谢综合征的相关性。方法:选择2015年3月至2016年5月于青岛市市立医院就诊的109例哮喘患者(A组)、102例代谢综合征患者(MS组)、73例哮喘合并代谢综合征患者(A＋MS组)和89名健康对照者(NC组)进行横断面研究，应用四引物扩增受阻突变体系PCR法检测所有试验对象的Apelin基因rs2235306位点基因型，酶联免疫吸附试验法检测血浆Apelin浓度，同时测定所有试验对象的体质量指数、血压、空腹血糖和肺功能。结果:(1)在女性群体，A组CC基因型和C等位基因频率较NC组显著升高(n χ2＝5.148、4.283，n P值均<0.05)，A＋MS组CC基因型和C等位基因频率较NC组显著升高(n χ2＝4.933、5.402，n P值均<0.05)，MS组CC基因型较NC组显著升高(n χ2＝4.604，n P＝0.032)；(2)A组、MS组和A＋MS组血浆Apelin水平均高于健康对照组(n P值均<0.05)；(3)在女性群体，MS组、A组和A＋MS组TT＋TC基因型血浆Apelin水平高于CC基因型(n t＝2.28、2.21、2.88，n P值均<0.05)；在男性群体，MS组、A组和A＋MS组T基因型血浆Apelin水平高于C基因型(n t＝2.48、2.61、2.45，n P值均<0.05)。n 结论:Apelin基因rs2235306位点多态性和代谢综合征及哮喘的发病具有相关性，其中C等位基因可导致Apelin基因的相对低表达，从而促进代谢综合征和哮喘的发病，是代谢综合征和哮喘的共同遗传易感基因。“,”Objective:To investigate the relationship of polymorphism of Apelin gene rs2235306 with bronchial asthma (asthma) and metabolic syndrome.Methods:From March 2015 to May 2016, 109 asthma patients (A group), 102 metabolic syndrome patients (MS group), 73 asthma combined metabolic syndrome patients (A+ MS group) and 89 normal controls (NC group) were collected in Qingdao Municipal Hospital.The genotypes of Apelin gene rs2235306 were analyzed by the method of tetra-primer amplification refractory mutation system PCR, the serum Apelin level was measured by enzyme-linked immuno sorbent assay, and statistcs of each subject′s body mass index blood pressure, fasting blood glucose and lung function were collected.Results:(1)For women, there were significant differences in genotype and allele frequency in Apelin gene rs2235306 between A group and NC group (n χ2=5.148, 4.283, both n P<0.05), there were also significant differences in genotype and allele frequency between A+ MS group and NC group (n χ2=4.933, 5.402, both n P<0.05). The frequency of CC genotype in women with metabolic syndrome was significantly higher than that in healthy women (n χ2=4.604, n P=0.032). (2)The serum Apelin levels of A group, MS group and A+ MS group were higher than that of NC group (all n P<0.05). (3)For women in MS group, A group and A+ MS group, the serum Apelin levels of TT+ TC genotype were higher than CC genotype (n t=2.28, 2.21, 2.88, all n P<0.05). For men in MS group, A group and A+ MS group, the serum Apelin levels of T genotype were higher than C genotype (n t=2.48, 2.61, 2.45, all n P<0.05).n Conclusions:Apelin gene rs2235306 polymorphism is correlated with metabolic syndrome and asthma.By down regulating expression of Apelin, the C allele may be the genetic factor that contribute to individual susceptibility for metabolic syndrome and asthma.