由外植体消毒灭菌产生了无菌苗,再从少量试管苗变成大量试管苗,从一瓶发展到几十瓶,甚至成千上万瓶,这就叫试管苗的增殖。试管苗的增殖主要有两种途径:一种是外植体先长出不分化的愈伤组织,再促进愈伤组织的培殖,而后从愈伤组织上分化出不定芽、胚状 Sterile sterilization by explants produced aseptic seedlings, and then a small amount of test tube into a large number of test tube seedlings, developed from a bottle to dozens of bottles, or even thousands of bottles, which is called the proliferation of test tube seedlings. There are mainly two ways for the proliferation of in vitro plantlets: one is that the explants firstly grow undifferentiated callus, then promote callus culture, and then differentiate into adventitious buds and embryoids from callus