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为研究花生开花下针期生长素在植株中的运输与分布规律,以‘中花4号’花生为试验材料,以14C-IAA为示踪物,对花生不同部位中14C-IAA的分布进行放射自显影定位分析,并利用液体闪烁计数仪进行定量检测。结果表明:涂布于花生主茎顶叶的14C-IAA在处理48h后向根和茎中的转运量增加,向下运输的速率约为5mm·h-1。通过浸泡处理进入根系的14C-IAA具有向顶传导的能力,但在根系中的滞留量较高,处理24h时14C-IAA向地上部分转运的能力较强。涂布于果针着生部位上方茎段的14C-IAA可同时向上、下两个方向运输,且处理24h时运输能力较强。带有果针的花在涂布处理72h后其14C-IAA主要向下运输;而尚未形成果针的花涂布处理后其14C-IAA运输至不同部位的含量为:叶>茎>根。
In order to study the transport and distribution of hypocotyl auxin in peanut during flowering, the distribution of 14C-IAA in different parts of peanut was determined using 14C-IAA as tracer with ’Zhonghua 4’ as test material. Autoradiography localization analysis, and the use of liquid scintillation counter for quantitative detection. The results showed that the translocation of 14C-IAA to the roots and stems of peanut main stem increased 48 hours after treatment, and the rate of downward transportation was about 5 mm · h-1. The 14C-IAA entering the root system by immersion treatment had the ability to conduct to the top, but had a higher retention in the root system, and the ability of 14C-IAA to transport to the aerial part was stronger at 24h. The 14C-IAA coated on the stem above the stalks of the fruit needles can be transported both upwardly and downwardly at the same time, and the transport capacity of the 14C-IAA is better when it is processed for 24h. The flowers with fruit needles were mainly transported downward after 72 hours of coating treatment. However, the 14C-IAA content of flowers with fruit needles transported to different parts after the flowers were not formed was leaf> stem> roots.