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本实验采用荧光分光光度法，以炎症介质５－羟色胺（５－ＨＴ）为指标，对脾虚型溃疡性结肠炎大鼠血浆进行分析，并同时观察了自制的补脾止泻冲剂和补脾益肠丸两种方药对实验性脾虚型ＵＣ大鼠血浆中５－ＨＴ的影响，探讨其治疗机理。结果发现，两种方药均有降低血浆中５－ＨＴ的作用，并和造型对照组相比较Ｐ＜０．０５，提示了补脾止泻冲剂治疗ＵＣ与降低血浆中５－ＨＴ有一定关系，同时说明了５－ＨＴ可作为ＵＣ炎症程度的辅助指标。
In this experiment, fluorescence spectrophotometry was used to measure serotonin (5-HT) as an indicator of inflammation in spleen-deficiency ulcerative colitis in rats. Simultaneous observation of self-made Bupi Zhixie Granules and Bupiyi The effect of two kinds of prescriptions of intestinal pills on 5-HT in plasma of experimental spleen-deficiency UC rats was explored. The results showed that both prescriptions had a role in lowering plasma 5-HT, and compared with the model control group P<0.05, suggesting that Bupi Zhixie Granules treatment of UC has a certain relationship with plasma 5-HT. It also shows that 5-HT can be used as an auxiliary indicator of UC inflammation.