论文部分内容阅读
【目的】探讨步长脑心通对脑缺血的保护作用机制。【方法】选用Wistar大鼠,随机分为正常组、假手术组、模型组与治疗组,再分为12 h和24 h 2个亚组,治疗组给予步长脑心通灌胃(剂量为0.45 g.kg-1.d-1);采用大脑中动脉线栓法复制大鼠脑缺血再灌注模型,应用免疫组织化学SP法检测大鼠脑组织一氧化氮合酶iNOS、eNOS的表达,采用图像分析仪测定光密度值,光镜下分析阳性细胞百分率。【结果】与模型组比较,治疗组iNOS表达显著降低(P<0.05),eNOS表达显著增高(P<0.05)。【结论】步长脑心通能降低脑缺血再灌注大鼠脑组织iNOS表达、增强eNOS表达,从而抑制神经细胞凋亡,发挥脑保护作用。
【Objective】 To investigate the protective effect of nao xin tong on cerebral ischemia. 【Methods】 Wistar rats were randomly divided into normal group, sham operation group, model group and treatment group. The rats were divided into 2 subgroups at 12 and 24 hours. 0.45 g.kg-1.d-1). The cerebral ischemia-reperfusion model was induced by middle cerebral artery occlusion in rats. The expressions of iNOS and eNOS were detected by immunohistochemical SP method , The optical density was measured by image analyzer, and the percentage of positive cells was analyzed by light microscope. 【Results】 Compared with the model group, the iNOS expression in the treatment group was significantly decreased (P <0.05) and the eNOS expression was significantly increased (P <0.05). 【Conclusion】 Buchangnaoxintong could reduce the expression of iNOS and increase the expression of eNOS in brain tissue of rats with cerebral ischemia-reperfusion, thus inhibiting the apoptosis of nerve cells and playing a neuroprotective role.