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为了逐步阐明中国人ＩＦＮ－α基因结构与功能的特点，将从一中国汉族正常胎儿肝细胞染色体ＤＮＡ中获得的一组ＰＣＲ克隆产物（ＰＣＲＡ０１、ＰＣＲＡ０２）分别进行核苷酸序列测定。结果表明，以上两次ＰＣＲ产物序列完全相同，它们与过去分离的ＩＦＮ－α１和ＩＦＮ－αＤ基因之间的氨基酸序列差异小于１％，为ＩＦＮ－α１基因的不同变种。ＰＣＲＡ０１、ＰＣＲＡ０２开放读码框架完全相同，与ＩＦＮ－α１和ＩＦＮ－αＤ基因相比较，其第２９９位为Ｔ，与其相应编码的第１００位氨基酸为Ｖａｌ。该基因为继黎孟枫等１９９１年所分离的ＩＦＮ－α１Ｃ基因之后所发现的又一中国人α１型干扰素基因新变种——ＩＦＮ－α１／１００Ｖ，建议命名为ＩＦＮ－α１ｄ基因。将该基因克隆于原核表达载体ＰＢＶ２２０，测定其抗病毒活性为２．４×１０７Ｕ／Ｌ。为进一步研究ＩＦＮ－α１亚型的生物学特性和其在基因进化上的关系奠定了基础
In order to clarify the characteristics of Chinese IFN-α gene structure and function, a series of PCR clones (PCRA01, PCRA02) obtained from chromosomal DNA of a Chinese Han normal fetal liver cells were sequenced respectively. The results showed that the sequences of the two PCR products were identical. The amino acid sequence differences between the two isolated PCR products were less than 1%, which were different variants of the IFN-α1 gene. PCRA01, PCRA02 open reading frame identical, compared with the IFN-α1 and IFN-αD gene, the 299th position is T, and its corresponding encoded amino acid 100 is Val. This gene is named as IFN-α1d gene after IFN-α1C gene isolated in 1991 by Leimeng Feng et al., A new Chinese variant of IFN-α1 / 100V discovered by Chinese. The gene was cloned into prokaryotic expression vector PBV220 and its antiviral activity was determined to be 2.4 × 107U / L. It laid the foundation for further study on the biological characteristics of IFN-α1 subtype and its relation to gene evolution