Simultaneous pyridine biodegradation and nitrogen removal were successfully achieved in a sequencing batch reactor (SBR) based on aerobic granules.In a typical SBR cycle,nitritation occurred obviously after the majority of pyridine was removed,while denitrification occurred at early stage of the cycle when oxygen consumption was aggravated.The effect of several key operation parameters,i.e.,air fiow rate,influent NH4+-N concentration,influent pH and pyridine concentration,on nitritation,pyridine degradation and total nitrogen (TN) removal,was systematically investigated.The results indicated that high air flow rate had a positive effect on both pyridine degradation and nitritation but a negative impact of overhigh air flow rate.With the increase of NH4+ dosage,both nitritation and TN removal could be severely inhibited.Slightly alkaline condition,i.e.,pH 7.0-8.0,was beneficial for both pyridine degradation and nitritation.High pyridine dosage often resulted in the delay of both pyridine degradation and nitritation.Besides,extracellular polymeric substances production was affected by air flow rate,NH4+ dosage,pyridine dosage and pH.In addition,high-throughput sequencing analysis demonstrated that Bdellovibrio and Paracoccus were the dominant species in the aerobic granulation system.Coexistence of pyridine degrader,nitrification related species,denitrification related species,polymeric substances producer and self-aggregation related species was also confirmed by highthroughput sequencing.