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目的:为了解肉鸡胴体中沙门氏菌的污染状况、多重耐药状况及耐药程度,对样品中的沙门氏菌进行分离鉴定和药敏试验研究。方法:根据GB/T 4789—2008对沙门氏菌进行分离鉴定,采用微量肉汤稀释法测定分离株的耐药性。结果:肉鸡胴体沙门氏菌的检出率高达34.8%;样品中分离的沙门氏菌血清型主要为奥尔巴尼型(29.6%)和印第安纳型(50.7%);药敏试验显示,大多数沙门氏菌至少对3种以上的抗生素产生耐药,耐3～7种药物的有24株(33.8%),耐8～12种药物的有19株(26.8%),耐13～16种药物的有26株(36.6%);分离株对抗生素的耐药情况是:萘啶酸(100%)、磺胺嘧啶(98.6%)、氨苄青霉素(97.2%)、复方磺胺甲恶唑(85.9%)、四环素(85.9%)和阿莫西林/克拉维酸(84.5%)。结论:肉鸡胴体中沙门氏菌的污染现象严重,沙门氏菌耐药或多重耐药现象十分普遍,未来应加强对鸡胴体中沙门氏菌感染及耐药的监测。
OBJECTIVE: To understand the status of Salmonella contamination, multi-drug resistance and drug resistance in broiler carcasses, Salmonella in samples was isolated and identified and drug susceptibility test was carried out. Methods: Salmonella was isolated and identified according to GB / T 4789-2008, and the antibiotic resistance of the isolated strains was determined by micro broth dilution method. Results: The detection rate of carcass Salmonella in broilers was as high as 34.8%. The serotypes of Salmonella isolated from samples were mainly Albainin type (29.6%) and Indiana type (50.7%). Susceptibility tests showed that most Salmonella were resistant to at least three 24 (33.8%) were resistant to 3 to 7 drugs, 19 (26.8%) were resistant to 8 ~ 12 drugs and 26 (36.6%) were resistant to 13 ~ 16 drugs. Resistant isolates were resistant to antibiotics: nalidixic acid (100%), sulfadiazine (98.6%), ampicillin (97.2%), trimethoprim sulfamethoxazole (85.9%), tetracycline Xilin / clavulanic acid (84.5%). Conclusion: The contamination of Salmonella in carcass of chicken is serious. Salmonella resistance or multidrug resistance is very common, and the surveillance of Salmonella infection and drug resistance in chicken carcass should be strengthened in the future.