目前人体乙型肝炎疫苗都是用乙型肝炎病毒的表面抗原(HBsAg)来制备的。有几种哺乳动物细胞株(例如人体肝细胞瘤、感染SV40病毒的猴肾细胞以及小鼠L细胞等)与细菌可以表达此种抗原。但是前者可能致癌,后者表达甚微。有的用DNA重组术获得的疫苗其抗原效价以及对人体的完全性与效用均不肯定。1982年Valenzuela等首次报道酵母菌细胞不仅可表达HBsAg基因,而且可以把多肽装配成与人体血浆中分离者完全相同的颗粒。为此,作者采用啤酒酵母的发酵液来产生adw亚型的HBsAg。此菌携带用酵母乙醇脱氢酶Ⅰ为启动子的可表达的基因载 Currently, human hepatitis B vaccines are prepared using the hepatitis B virus surface antigen (HBsAg). There are several mammalian cell lines (such as human hepatoma, monkey kidney cells infected with SV40 virus and mouse L cells, etc.) and bacteria can express this antigen. However, the former may cause cancer, while the latter express little. Some vaccines obtained by DNA recombination antigen titer and the completeness and effectiveness of the human body are not sure. In 1982, Valenzuela et al. First reported that yeast cells can not only express HBsAg gene, but also assemble peptides into the same granules as human plasma separator. For this reason, the authors used the brewer’s yeast fermentation broth to produce the adw subtype of HBsAg. This bacterium carries an expressible gene cassette containing yeast alcohol dehydrogenase I as a promoter