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目的:观察黄荆子乙酸乙酯提取物(EVn-50)抑制人急性髓性白血病HL-60细胞生长和诱导凋亡作用。方法:体外培养HL-60细胞和人外周血单核细胞(PBMC),MTT法测定细胞增殖;软琼脂培养集落形成法检测细胞生长;PI染色流式细胞术分析细胞凋亡。结果:EVn-50对HL-60细胞增殖具有显著抑制作用,呈浓度依赖性;而对PBMC的增殖活性影响小。EVn-50对HL-60细胞集落形成有抑制效应,呈浓度依赖性。EVn-50有效诱导HL-60细胞凋亡。结论:EVn-50具有抑制HL-60细胞生长和诱导凋亡作用。
OBJECTIVE: To observe the effect of ethyl acetate extract (EVn-50) against the growth and apoptosis of human acute myeloid leukemia HL-60 cells. METHODS: HL-60 cells and human peripheral blood mononuclear cells (PBMCs) were cultured in vitro. Cell proliferation was measured by MTT assay; cell growth was detected by soft agar culture colony formation assay; apoptosis was analyzed by PI staining flow cytometry. RESULTS: EVn-50 had a significant inhibitory effect on the proliferation of HL-60 cells in a concentration-dependent manner, but had little effect on the proliferative activity of PBMCs. EVn-50 has an inhibitory effect on colony formation of HL-60 cells in a concentration-dependent manner. EVn-50 effectively induces apoptosis in HL-60 cells. Conclusion: EVn-50 can inhibit the growth of HL-60 cells and induce apoptosis.