本研究采用MTT法对海洋放线菌、细菌、霉菌及极地和大洋细菌进行细胞毒活性物质的筛选 ,结果有 1 0 %放线菌及一株极地细菌具有细胞毒活性 .此外利用DNA修复特性在E .coli 3 4 3 /591和E .coli 3 4 3 /63 6之间的差异性 ,对前面筛得的菌株进行DNA损伤的筛选 ,结果得到两株活性菌株 .采用荧光染色观察得到 3株具有诱导肿瘤细胞凋亡的菌株 ,这些具抗肿瘤活性的菌株可供进一步研究 . In this study, the cytotoxic activity of marine actinomycetes, bacteria, molds, and polar and oceanic bacteria was screened by MTT assay. The results showed that 10% actinomycetes and one polar bacteria had cytotoxic activity. In addition, DNA repair properties were used. Based on the difference between E.coli 3 4 3 /591 and E.coli 3 4 3 /63 6 , the screened strains were screened for DNA damage. Two strains of active strains were obtained and observed by fluorescent staining. 3 The strain has a strain that induces apoptosis of tumor cells. These strains with anti-tumor activity can be further studied.