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大鼠在戊巴比妥麻醉下,通过气管切开通气,以便测定潮气量及呼吸道气压。全部动物均用~(125)碘标记白蛋白和~(99)锝标记红血球,以测定肺微血管渗漏;并分作5组:(Ⅰ)间歇正压通气(IPPV),吸气压力峰值7cmH_2O,潮气量13±3ml/kg;Ⅱ)高潮气量(HiV,40±3ml/kg)及高充气压(HiP,45cmH_2O);(Ⅲ)通气压峰值同第Ⅱ组;加用10cmH_2O的PEEP(潮气量为25±1ml/kg),Ⅳ)充气压为45cmH_2O的低潮气量(LoV,19±1ml/kg);(Ⅴ)负压吸气及高潮气量。各组肺脏均制成标本,在显微镜下作解剖学研究。动物在实验期间无死亡;但第Ⅴ组(Lop-HiV)皆呈垂死状态。第Ⅱ及第Ⅴ组(HiP-HiV及LoP-HiV)气管内出现水肿液体,肺部明
Rats were ventilated by tracheotomy under pentobarbital anesthesia in order to determine tidal volume and airway pressure. All animals were labeled with 125I-albumin and 99Tc-labeled erythrocytes to detect pulmonary microvascular leakage. The animals were divided into 5 groups: (I) intermittent positive pressure ventilation (IPPV), inspiratory pressure peak of 7cmH2O , Tidal volume 13 ± 3ml / kg; Ⅱ) high tidal volume (HiV, 40 ± 3ml / kg) and high inflation pressure (HiP, 45cmH2O); (Ⅲ) Volume of 25 ± 1 ml / kg), IV) low tidal volume (LoV, 19 ± 1 ml / kg) for inflation pressure of 45 cm H 2 O, and (V) negative suction and high tidal volume. Each group of lungs were made specimens, under the microscope for anatomical study. Animals did not die during the experiment; but Group V (Lop-HiV) showed dying. In Group II and Group V (HiP-HiV and LoP-HiV), edema fluid appeared in the trachea,