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用恙虫病立克次体感染小鼠使其产生腹水，取腹水原液与部分提纯的恙虫病立克次体悬液加或不加二甲基亚砜、采用四级或二级降温方法作液氮保存，并分别于保存１、３、６和１２个月后取出作形态观察及感染小鼠能力测定。结果证明恙虫病立克次体经液氮保存１年后仍具有对小鼠的感染能力。其中以腹水原液，加二甲基亚砜，采用二级降温法作液氮保存效果较好。采用液氮保存恙虫病立克次体具有节时省物、可靠程度高的优点，为对恙虫病立克次体的生物特性和恙虫病的预防、诊断和治疗作深入研究奠定了基础
Rickettsia tsutsugamushi infection with mice to make it ascites, ascites fluid and part of the purified tsutsugamushi rickettsial suspension with or without dimethyl sulfoxide, using four or two cooling method for the liquid Nitrogen and stored for 1, 3, 6, and 12 months after storage for morphological observation and determination of the ability of the infected mice. The results show that the tsutsugamushi Rickettsia liquid nitrogen after 1 year still has the ability to infect mice. Which ascites fluid, plus dimethyl sulfoxide, the use of two cooling method for liquid nitrogen preservation is better. The use of liquid nitrogen to save scrub typhus Rickettsia has the advantages of saving time and money, high reliability, in order to scrub typhus Rickettsia biological characteristics and scrub typhus prevention, diagnosis and treatment for further study laid the foundation