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目的利用原子力显微镜(AFM)观察支撑磷脂膜的微观形成过程。方法冰浴超声法制备磷脂脂质体溶液,囊泡融合法形成人工磷脂双分子层,用AFM进行观察。结果利用AFM观察到磷脂膜的微观形成过程为:脂质体囊泡吸附→脂质体囊泡之间发生黏着→脂双层之间形成接触点→磷脂囊泡破裂→形成磷脂分子层→磷脂分子层间流动进行融合形成大片磷脂膜。结论 AFM可观察磷脂膜的微观形成过程,进而为细胞膜和膜蛋白的功能研究提供基础。
Objective To observe the microscopic formation of phospholipid membrane by atomic force microscopy (AFM). Methods The phospholipid liposomes were prepared by the ice bath ultrasonic method. The artificial phospholipid bilayers were formed by vesicle fusion method and observed by AFM. Results The microscopic formation process of phospholipid membrane was observed by AFM: liposome vesicle adsorption → adhesion between liposome vesicles → contact point between lipid bilayers → rupture of phospholipid vesicles → formation of phospholipid molecular layer → phospholipid Molecular fluid flow between the fusion to form a large phospholipid membrane. Conclusion AFM can observe the microscopic formation process of phospholipid membrane, and then provide the basis for the study of the function of cell membrane and membrane protein.