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试验采用10%和15%两种 PEG 溶液浓度,比较了三个抗旱性不同品种的小麦幼苗在水分胁迫期间可溶性糖、蔗糖、有机酸和氨基酸等渗透调节物质的动态变化和积累差异。结果表明,水分胁迫初期可溶性糖及蔗糖含量下降、然后上升、积累,并且同一品种10% PEG 胁迫可溶性糖总量积累高于15%PEG胁迫,蔗糖积累情况相反。糖分积累的程度存在着品种间差异。PEG 引起的水分胁迫使科拜和祥云爬地麦的有机酸总量有不同程度降低,云麦29则有不同程度增加。氨基酸总量分析揭示,抗性品种比敏感品种积累更多的氨基酸。试验还观察到,15%PEG 胁迫能迅速导致三个品种的 Leu+Phe 的积累,而10%PEG 胁迫的积累情况随品种而异。此外,还比较分析了脯氨酸、天冬氨酸、天冬酰胺,谷氨酸,异亮氨酸等个别氨基酸在不同品种,不同胁迫以及不同时间进程中的消长变化。水分胁迫引起的溶质积累,在种类,数量和速率方面存在着明显的品种间差异。与干旱敏感品种相比,抗性品种具有更强的渗透调节能力。
The concentrations of two PEG solutions, 10% and 15%, were used to compare the dynamic changes and accumulation of soluble sugar, sucrose, organic acids and amino acids in wheat seedlings of three drought-resistant cultivars under water stress. The results showed that soluble sugar and sucrose content decreased, then increased and accumulated in the early stage of water stress, and the accumulation of soluble sugar under 10% PEG stress in the same variety was higher than that under 15% PEG stress. The accumulation of sucrose was opposite. The degree of sugar accumulation there is a difference between varieties. The water stress caused by PEG reduced the total amount of organic acids in Kobay and Cheung Wan climatic wheat to varying degrees, and Yunmai 29 increased in varying degrees. Total amino acid analysis revealed that resistant varieties accumulated more amino acids than susceptible varieties. It was also observed that 15% PEG stress rapidly led to the accumulation of Leu + Phe in three cultivars, whereas the accumulation of 10% PEG stress varied with the cultivar. In addition, the changes of proline, aspartic acid, asparagine, glutamic acid, isoleucine and other amino acids in different varieties, different stresses and different time course were compared. Solute accumulation caused by water stress has obvious inter-species differences in species, quantity and rate. Resistant varieties have stronger osmotic regulation than drought-sensitive cultivars.