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目的：分析确定人Ｆａｓ（Ｈ－Ｆａｓ）抗原具有死亡效应的表位区域。方法：采用可及性方案、可塑性方案，结合抗原性方案、二级结构方案预测Ｈ－Ｆａｓ抗原Ｂ细胞表位。Ｆｍｏｃ固相合成法合成部分预测表位肽段并鉴定后，经固相ＥＬＩＳＡ确定是否与具有诱导细胞凋亡（ＰＣＤ）的抗人ＦａｓｍＡｂ２Ａｌ、ＤＸ２、ＣＨ－１１反应。结果：Ｈ－Ｆａｓ抗原胞外区的Ｂ细胞表位可能位于Ｎ－末端氨基酸（ＡＡ）残基５１～７８，１０２～１１０，１４５～１５７等区域内或它们附近。用Ｆｍｏｃ固相合成法合成Ｎ－末端ＡＡ残基２～１５，５０～８３肽段，毛细管电泳鉴定合成效率，ＡＡ序列分析仪检测合成序列组成正确后，经固相ＥＬＩＳＡ法检测，抗人ＦａｓｍＡｂ２Ａ１、ＤＸ２、ＣＨ－１１均不识别Ｎ－末端１００个ＡＡ内肽段。结论：Ｈ－Ｆａｓ抗原死亡表位可能界定在１００～１５７区域。
OBJECTIVE: To analyze epitope regions that determine the death effect of human Fas (H-Fas) antigens. METHODS: The B cell epitopes of H-Fas antigen were predicted using the accessibility protocol, the plastid protocol, the antigenic protocol, and the secondary structure protocol. Fmoc solid-phase synthesis of part of the predicted peptide epitopes identified and identified by solid-phase ELISA to determine whether with the induction of apoptosis (PCD) anti-human FasmAb2Al, DX2, CH-11 reaction. Results: The B cell epitopes in the extracellular region of H-Fas antigen may be located in or near the N-terminal amino acid residue (AA) residues 51-78,102-110,145-157. The N-terminal AA residues 2 ~ 15 and 50 ~ 83 were synthesized by Fmoc solid phase synthesis. The synthetic efficiency was identified by capillary electrophoresis. The composition of the synthesized sequence was confirmed by AA sequence analyzer. After being detected by solid-phase ELISA, anti-human FasmAb2A1 , DX2, CH-11 did not recognize N-terminal 100 AA internal peptide. Conclusion: The death epitope of H-Fas antigen may be defined in the region of 100-157.