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以6个不同基因型的玉米自交系幼嫩叶段为外植体,研究其愈伤诱导、继代和分化过程。结果表明,3mg/L 2,4-D是诱导叶段愈伤组织较为适宜的,玉米愈伤组织的诱导及分化与基因型有密切关系。从供试的6个基因型中筛选出出愈率较高、愈伤组织形态较好的3个基因型齐319、Mo17、鲁原92,对它们的愈伤组织进行GUS瞬时表达检测表明,来源于幼苗叶段的愈伤组织GUS+转化率较高。
Six young corn inbred lines with different genotypes were used as explants to study their callus induction, subculture and differentiation process. The results showed that 3 mg / L 2,4-D was more suitable for inducing leaf segment callus. The induction and differentiation of maize callus were closely related to genotype. Three genotypes Qi 319, Mo17 and Luyuan 92 were screened out from the six genotypes tested for their higher callus and callus morphology. The transient expression of GUS in their callus was detected, The callus from the leaf segments of seedlings had higher GUS + conversion rate.