目的制备分泌血吸虫抗原的杂交瘤。方法采用杂交瘤技术，将血吸虫成虫细胞与Ｓｐ２／０骨髓瘤细胞融合，ＨＡＴ选择性培养。结果获得了１株培养上清能与血吸虫病人血清起反应的杂交瘤，其染色体数为７２～８４，该杂交瘤细胞可持续培养１１周以上，但其生长缓慢，在培养８周后，出现细胞逐渐死亡。结论杂交瘤技术不适于远缘杂交，细胞死亡可能与ＤＮＡ隔离和染色体物质丢失有关 Objective To prepare a hybridoma secreting schistosome antigen. Methods The hybridoma technique was used to fuse Schistosoma japonicum adult cells with Sp2 / 0 myeloma cells and HAT selective culture. Results One hybridoma with the culture supernatant that could react with the serum of schistosomiasis patients was obtained. The number of chromosomes was 72-84. The hybridoma cells could be cultivated continuously for more than 11 weeks, but its growth was slow. After 8 weeks of culture, it appeared Cells gradually die. Conclusion Hybridoma technology is not suitable for distant hybridization, cell death may be related to DNA isolation and chromosomal loss