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以中药材 贯叶金丝桃的提取物为对象 ,对其主要有效成分黄酮类化合物总含量测定的几种分光光度法进行了比较研究。发现文献中某些常用的分光光度法存在严重的缺陷。通过与高效液相色谱法测定结果比较 ,建立了一种测定总黄酮含量的分光光度法
A comparative study of several spectrophotometric methods for the determination of total flavonoids, the main active ingredient, was conducted for the extracts of Hypericum perforatum. Some of the commonly used spectrophotometric methods found in the literature have serious drawbacks. By comparing with the result of high performance liquid chromatography, a spectrophotometric method for determination of total flavonoids