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利用固相微萃取-气质联用和内标定量技术建立了水体和斑马鱼体内土臭素(Gsm)和二甲基异冰片(2-Mib)的分析方法,并利用该方法研究水体中Gsm和2-Mib在斑马鱼体内的富集和暂养效果。结果表明,水体中不同浓度Gsm和2-Mib的添加回收率范围为97.2%~109.9%和88.5%~101.6%,斑马鱼中不同浓度Gsm和2-Mib的添加回收率范围为78.5%~87.2%和88.3%~91.4%;水体中不同浓度的Gsm和2-Mib能在鱼体内较快富集,鱼体内Gsm和2-Mib浓度和水体中的浓度成正相关;鱼体内Gsm和2-Mib的浓度和暂养时间成反相关,但降低较慢。水体中的Gsm和2-Mib通过鱼鳃和体表迅速富集到鱼体中,成为鱼体土腥味来源的主要外源因子,清水暂养对土腥味物质Gsm和2-Mib的去除有一定作用,但仍需配合其他去除方法使用效果更佳。
The analytical method of geosmin (Gsm) and dimethylisoborneol (2-Mib) in water and zebrafish was established by solid-phase microextraction-gas chromatography-mass spectrometry and internal standard quantification. The method was applied to study the effects of Gsm and 2-Mib in zebrafish body enrichment and holding effect. The results showed that the recoveries ranged from 97.2% to 109.9% and from 88.5% to 101.6% for Gsm and 2-Mib in water, and the recoveries of Gsm and 2-Mib in zebrafish ranged from 78.5% to 87.2 % And 88.3% -91.4%, respectively. Gsm and 2-Mib concentrations in water were faster than those in water, and the concentration of Gsm and 2-Mib in fish was positively correlated with the concentration of Gsm and 2-Mib The concentration and holding time are inversely related, but the decrease is slower. Gsm and 2-Mib in water body rapidly enriched into the fish body through fish gills and body surface, which became the main exogenous factors of fishy soil origin. The removal of Gsm and 2-Mib Have a certain effect, but still need to cooperate with other removal methods to use better effect.