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本文作者报道了由于使用一种新的反应瓶从而改进了目前采用的Magos法检测人血、尿和组织总汞与无机汞的技术。使用这种反应瓶可以使测定样品的数量增加二倍,样品及试剂的需用量减少到原来的五分之一,方法的精密度、准确度和灵敏度都显著地提高,并可以自动分析。 [试剂] 全部试剂均为分析纯。按下法用去离子水配制试剂。 氯化汞标准溶液:称取0.6767克氯化汞溶于1升5％(W/V)硫酸中制成贮备液,取此液1ml溶解在含有9克氯化钠,0.7545克EDTA和0.1克L一半胱氨酸的水溶液中稀释至1升,最终溶液为0.5μg/ml汞,如冷冻保存可稳定6个月。取1ml贮备液用去离子水稀释至50毫升制备成10ng/ml汞
The authors report that the technique of detecting Magos method for total and in-vivo mercury in human blood, urine and tissue has been improved due to the use of a new reaction flask. The use of such a reaction flask can double the number of test samples and reduce the sample and reagent requirements by a factor of five. The precision, accuracy and sensitivity of the method are significantly improved and can be automatically analyzed. [Reagents] All reagents are of analytical grade. Press method to prepare reagent with deionized water. Mercuric chloride standard solution: Weigh 0.6767 g of mercuric chloride in 1 liter of 5% (w / v) sulfuric acid to prepare a stock solution. Take 1 ml of this solution in a solution containing 9 g of sodium chloride, 0.7545 g of EDTA and 0.1 g L-cysteine in aqueous solution to 1 liter, the final solution of 0.5μg / ml mercury, such as cryopreservation can be stable for 6 months. Take 1ml stock solution diluted to 50ml with deionized water to prepare 10ng / ml mercury