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目的：提取并纯化豚鼠脑、脊髓髓鞘碱性蛋白（ＭＢＰ），对其理化特征和致实验性自身免疫性脑脊髓炎（ＥＡＥ）的活性进行鉴定，并初步探讨口服ＭＢＰ诱导免疫耐受防治ＥＡＥ的作用。方法：以豚鼠脑、脊髓为原料，通过去脂、抽洗、酸溶解、高速离心等步骤提取ＭＢＰ，并在Ｗｉｓｔａｒ大鼠中诱导ＥＡＥ和口服耐受。结果：所提取的ＭＢＰ具有与文献报告结果类似的理化特征和诱发ＥＡＥ的活性；通过口服ＭＢＰ，成功诱导出对ＥＡＥ的免疫耐受。结论：ＭＢＰ对研究ＥＡＥ的发病机制和病理变化及通过口服ＭＢＰ诱导免疫耐受对ＥＡＥ和多发性硬化症的防治具有重要意义。
OBJECTIVE: To extract and purify the myelin basic protein (MBP) from brain and spinal cord of guinea pig and to identify its physical and chemical characteristics and the activity of experimental autoimmune encephalomyelitis (EAE). To explore the preventive and therapeutic effect of oral MBP on immune tolerance The role of EAE. Methods: The guinea pig brain and spinal cord were taken as raw materials. MBP was extracted by degreasing, washing, acid dissolving and high speed centrifugation. EAE and oral tolerance were induced in Wistar rats. Results: The extracted MBP had similar physicochemical characteristics and EAE-induced activity as reported in the literature. The immune tolerance to EAE was successfully induced by oral administration of MBP. Conclusion: MBP plays an important role in the study of the pathogenesis and pathological changes of EAE and the induction of immune tolerance by oral MBP on the prevention and treatment of EAE and multiple sclerosis.