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目的:测定不同厂家银杏达莫注射液中的双嘧达莫、槲皮素、山柰素和异鼠李素的含量并进行比较。方法:采用HLPC法测定3个厂家的银杏达莫注射液中各主要成分的含量,以Agilent HC-C_(18)为色谱柱,流动相为乙腈-0.4%磷酸溶液(含0.1%三乙胺),采用梯度洗脱,检测波长为360nm,流速1.0mL·min~(-1),柱温30℃,进样量30μL。结果:不同厂家双嘧达莫和总黄酮醇苷的含量分别为0.382～0.404mg·mL~(-1)和0.951～1.053 mg·mL~(-1)两者均无统计学差异,但各黄酮醇苷元的含量有统计学差异。结论:不同厂家银杏达莫注射液由于制剂工艺、原材料产地等不同,导致了各黄酮醇苷元的含量差异较大。
OBJECTIVE: To determine and compare the dipyridamole, quercetin, kaempferol, and isorhamnetin levels in different manufacturers of ginkgo-damo extract. Methods: The HLPC method was used to determine the contents of the main components in the ginkgo dipyridamole injections of 3 manufacturers. The chromatographic column was Agilent HC-C_(18) and the mobile phase was acetonitrile-0.4% phosphoric acid solution containing 0.1% triethylamine. ), Gradient elution, detection wavelength of 360nm, flow rate 1.0mL · min -1, column temperature 30 °C, injection volume 30μL. RESULTS: There were no significant differences in the contents of dipyridamole and total flavonol glycosides from different manufacturers, which were 0.382-0.404 mg·mL-1 and 0.951-1.053 mg·mL-1, respectively. The flavonol aglycone content was statistically different. Conclusion: The different manufacturers of ginkgo-damo injection due to the preparation process, the origin of raw materials and other different, leading to a large difference in the content of flavonol aglycone.