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目的观察NADPH氧化酶抑制剂apocynin(APO)对心肌梗死后心衰大鼠白介素-1β(IL-1β)、去甲肾上腺素及心功能的影响。方法采用左冠状动脉前降支结扎术致心肌梗死制备大鼠心衰模型和假手术模型(Sham),连续4周经侧脑室给予NADPH氧化酶抑制剂apocynin(10μg/hr)或人工脑脊液干预(aCSF)。4周后,采用ELISA检测室旁核和血浆IL-1β的含量,采用高效液相色谱法测量血浆去甲肾上腺素水平,并测定全心/体重(WH/BW)、肺/体重(Lung/BW)。结果与Sham组比较,心衰大鼠室旁核和血浆IL-1β的含量明显增加(P<0.05);血浆去甲肾上腺素水平明显增加(P<0.05)。与HF+aCSF组相比,HF+APO组血浆增高的IL-1β和去甲肾上腺素的含量降低(P<0.05)、WH/BW和Lung/BW降低(P<0.05)。结论心肌梗死后心衰大鼠室旁核和血浆IL-1β的含量增加并伴有交感神经活动增加,从而加重心衰;抑制NADPH氧化酶的活性可以降低外周交感神经兴奋性和IL-1β的含量,并且可能通过该抑制作用对心功能改善及肺淤血减轻发挥作用。
Objective To investigate the effects of apocynin (APO), an inhibitor of NADPH oxidase, on interleukin-1β (IL-1β), norepinephrine and cardiac function in heart failure rats after myocardial infarction. Methods The model of heart failure and sham operation were induced by left anterior descending coronary artery ligation in rats. The rats were anesthetized with apocynin (10μg / hr) or artificial cerebrospinal fluid aCSF). Four weeks later, the contents of paraventricular nucleus and plasma IL-1βwere detected by ELISA, the plasma norepinephrine level was measured by high performance liquid chromatography, and the levels of WH / BW and Lung / BW). Results Compared with Sham group, the contents of paraventricular nucleus and plasma IL-1β in heart failure rats were significantly increased (P <0.05), and plasma norepinephrine levels were significantly increased (P <0.05). Compared with HF + aCSF group, the levels of plasma IL-1β and norepinephrine in HF + APO group were decreased (P <0.05), while WH / BW and Lung / BW were decreased (P <0.05). Conclusion The content of paraventricular nucleus and plasma IL-1β in heart failure rats after myocardial infarction increased with the increase of sympathetic activity, thus aggravating heart failure. Inhibition of NADPH oxidase activity could reduce the excitability of peripheral sympathetic nerve and IL-1β Content, and may play a role in improving cardiac function and reducing lung congestion by this inhibition effect.