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目的对大鼠进行股动脉血流介导的血管舒张功能(FMD)检测,建立标准化检测方法。方法 21只同周龄Sprague-Dawley(SD)大鼠,随机分为4组:高血流速度(HFV,取血流速度≥100mm/s的血管段测量)组(n=5)、低血流速度(LFV,取血流速度<100 mm/s的血管段测量)组(n=6)、一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(l-NAME)组(取血流速度≥100mm/s的血管段测量,同时注射l-NAME 8mg/kg,n=5),溶剂对照组(取血流速度≥100mm/s的血管段测量,同时注射生理盐水0.1mL/100g体质量,n=5),使用动物超声系统在血管封堵器压迫股动脉造成缺血5min后的0、60、120、180、240、300s分别测定不同时间点的FMD及血流速度,同时观察l-NAME注射对FMD的影响。结果缺血再灌注后5min内,HFV组与LFV组大鼠股动脉FMD在120s时达到峰值,高于l-NAME组FMD峰值[(16.49±2.23)%、(12.05±0.97)%比(6.30±1.04)%,均P<0.05],各组血流速度及管壁切应力(WSS)随时间增加逐渐降低(P_(趋势)<0.05)。与HFV组相比,l-NAME组WSS升高(P<0.05)。而溶剂对照组与HFV组在各项指标差异均无统计学意义。结论测定大鼠股动脉HFV段血管FMD是无创、高效而且可连续监测的一种内皮功能检测方法。
Objective To investigate the femoral artery blood flow-mediated vasodilation (FMD) in rats and to establish a standardized test method. Methods 21 Sprague-Dawley (SD) rats of the same age were randomly divided into 4 groups: high blood flow velocity (HFV, blood vessel velocity≥100mm / s) Flow velocity (LFV, blood vessel velocity <100 mm / s) (n = 6), nitric oxide synthase inhibitor l-NAME group The blood flow velocity≥100mm / s, while the injection of l-NAME 8mg / kg, n = 5), the solvent control group Body mass, n = 5). The animals were anesthetized to determine the FMD and blood flow velocity at different time points at 0, 60, 120, 180, 240 and 300 s after occlusion of the femoral artery by the vascular occlusion device To observe the impact of l-NAME injection on FMD. Results Within 5 minutes after ischemia-reperfusion, the FMD of femoral artery in HFV group and LFV group peaked at 120s, which was higher than that in l-NAME group [(16.49 ± 2.23)% vs (12.05 ± 0.97)% ± 1.04)%, all P <0.05]. The blood flow velocity and wall shear stress (WSS) decreased gradually with the increase of time (P_ (trend) <0.05). Compared with HFV group, WSS of l-NAME group increased (P <0.05). The solvent control group and HFV group in the various indicators were no significant difference. Conclusion The determination of vascular FMD in the femoral artery of the femoral artery is a noninvasive, highly effective and continuous monitoring of endothelial function.