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目的:检测乙肝感染孕妇的血清乙肝病毒标志物及所产新生儿脐血清HBV-DNA,探讨孕妇乙肝感染状况与新生儿宫内感染的关系,寻找有效阻断乙肝宫内感染的措施。方法:采用ELISA法检测302例乙肝感染孕妇血清乙肝病毒标志物,用Realtime-PCR法检测新生儿脐血清HBV-DNA。结果:“大三阳”和“小三阳”孕妇分别占64.90%和21.19%,“大三阳”组孕妇的新生儿脐血清HBV-DNA阳性率为46.88%,明显高于“小三阳”组的1.02%。孕妇血清HBeAg阳性组的新生儿脐血清HBV-DNA阳性率为46.27%,远高于阴性组的1.28%。结论:孕妇乙肝感染状况与新生儿宫内感染率密切相关,孕妇血清HBeAg可作为宫内感染发生的预测指标;HBeAg阳性妇女待HBeAg转阴后再妊娠,可大大减少宫内感染的发生。
OBJECTIVE: To detect the serum hepatitis B virus (HBV) markers of HBV-infected pregnant women and the cord blood serum HBV-DNA of newborns, and to explore the relationship between HBsAg status and neonatal intrauterine infection in pregnant women to find out an effective way to block intrauterine HBV infection. Methods: Serum HBV markers were detected by ELISA in 302 pregnant women with HBV infection. HBV-DNA of neonatal umbilical serum was detected by Realtime-PCR. Results: The positive rate of HBV-DNA in cord blood serum of pregnant women with “Sanshengyang” and “Sansangyang” accounted for 64.90% and 21.19% respectively, and that of “Sanshengyang” group was 46.88% In the “Little Sanyo” group of 1.02%. The positive rate of HBV-DNA in cord serum of pregnant women with HBeAg-positive group was 46.27%, much higher than that of negative group (1.28%). Conclusion: The status of hepatitis B infection in pregnant women is closely related to the intrauterine infection rate in newborns. Serum HBeAg in pregnant women can be used as a predictor of intrauterine infection; HBeAg-positive women can reduce the incidence of intrauterine infection after HBeAg negative pregnancy.