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以金线莲茎段为试验材料,得到无菌的诱导芽体。通过萌发的芽体上的茎尖和茎段分别诱导愈伤组织和原球茎,建立金线莲的无菌培养体系,筛选出金线莲组培快繁的最佳培养基配方。结果表明:金线莲茎段诱导芽体萌发最适宜培养基配方为MS+BA1.5mg/L+香蕉汁100g/L+NAA0.5mg/L,茎尖诱导原球茎最适宜培养基配方为MS+BA2.0mg/L+NAA0.3mg/L+HyponcexⅠ3.0g/L,原球茎继代增殖最适宜培养基配方为1/2MS+BA2.0mg/L+NAA0.3mg/L+香蕉汁100g/L+AC0.2%,愈伤组织诱导丛生芽最适宜培养基配方为1/2MS+BA2.0mg/L+NAA0.2mg/L+香蕉汁100g/L,丛生芽继代增殖培养最适宜培养基为1/2MS+BA3.0mg/L+NAA0.5mg/L+KT1.0mg/L+香蕉汁100g/L+AC0.2%,幼苗生根培养最适宜培养基配方为1/2MS+IBA1.0mg/L+NAA0.3mg/L+AC0.2%。
The goldenrod stems were used as the experimental materials to obtain sterile induced buds. The callus and the protocorm were induced respectively by shoot tips and stem segments on the germinated buds. The sterile culture system of Anoectochilus roxburghii was established and the best culture medium was selected. The results showed that the best medium for inducing germination was MS + BA1.5mg / L + banana juice 100g / L + NAA0.5mg / L, and the best medium for inducing shoots was MS + BA2.0mg / L + NAA0.3mg / L + HyponcexⅠ3.0g / L, the optimal culture medium of protocorm multiplication was1 / 2MS + BA2.0mg / L + NAA0.3mg / L + banana juice100g / L + AC0.2%, callus induction of clustered buds most suitable medium formula 1 / 2MS + BA2.0mg / L + NAA0.2mg / L + banana juice 100g / L, the most suitable medium for proliferation of clustered buds 1 /2MS+BA3.0mg/L+NAA0.5mg/L+KT1.0mg/L+ Banana juice 100g / L + AC0.2%, the most suitable culture medium for seedling rooting was 1 / 2MS + IBA1.0mg / L + NAA 0.3 mg / L + AC 0.2%.