【摘 要】
：
对普通小麦(Triticum aestivum)-节节麦(Aegilops squarrosa)八倍体(2n=8x=56,AABBDDDD)与硬粒小麦(Triticum durum)-簇毛麦(Haynaldia villosa)六倍体(2n=6x=42,AABBVV)杂交
【机 构】
：
南京农业大学农业部作物细胞遗传重点开放实验室
【基金项目】
：
美国McKnight Foundation;
论文部分内容阅读
对普通小麦(Triticum aestivum)-节节麦(Aegilops squarrosa)八倍体(2n=8x=56,AABBDDDD)与硬粒小麦(Triticum durum)-簇毛麦(Haynaldia villosa)六倍体(2n=6x=42,AABBVV)杂交后,将所得七倍体杂种(AABBDDV)进行连续自交,在F4代中利用C-分带鉴定出可能的簇毛麦6V二体附加系95-7和2V二体附加系26-7,其花粉母细胞染色体在减数分裂中期I的配对构型分别为0.14I+20.42 +1.50和o.10I+20.07 +1.82 ;进一步将95-7和26-7的基因组DNA用EcoRI酶切,分别用小麦族第6部分同源群短臂探针Psr113和第2部分同源群长臂探针BCD240进行Southem杂交,结果显示具有簇毛麦的特异杂交带,进一步确证了95-7和26-7分别是普通小麦-簇毛麦6V和2V二体附加系.
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