论文部分内容阅读
采用从鲤中分离出来的 32对微卫星DNA标记,对异源四倍体鲫鲤、红鲫和野鲤的基因组DNA进行了研究。在筛选出的 15对微卫星引物中,随引物不同,各等位基因数为 1 ~8个,大小在 100 ~420bp之间。从 3个不同群体内部的遗传相似系数来看,异源四倍体鲫鲤个体之间的遗传相似系数最大,说明异源四倍体鲫鲤群体内部的遗传变异程度最低,已经形成了一个遗传性状稳定的群体。从 3个不同群体之间的遗传相似系数来看,异源四倍体鲫鲤和红鲫遗传相似系数为 0 5625,和野鲤的遗传相似系数为 0 5125,说明异源四倍体鲫鲤接受原始母本的遗传物质比原始父本野鲤要多一些。微卫星标记与以前报道的RAPD标记的检测结果是相似的,然而由微卫星标记获得的种群内和种群间的遗传距离均大于RAPD,说明微卫星标记比RAPD标记显示出更高的个体多态性。
Genomic DNA of allotetraploid crucian carp, red crucian carp and wild carp was studied using 32 pairs of microsatellite DNA markers isolated from common carp. Among the 15 pairs of microsatellite primers screened, with the different primers, the number of alleles was 1 to 8 and the size was between 100 and 420 bp. Genetic similarity coefficient of three different populations showed that the genetic similarity coefficient between allotetraploid crucian carp individuals was the highest, indicating that the genetic variation within the allotetraploid crucian carp population was the lowest, and a genetic Stable group. The genetic similarity coefficient of three different populations showed that the genetic similarity coefficient of allotetraploid and red crucian carp was 0 5625 and the genetic similarity coefficient of wild carp was 0 5125, indicating that allotetraploid crucian carp The genetic material that accepts the original female parent is more than the original male wild carp. Microsatellite markers were similar to the previously reported RAPD markers, however, the genetic distance within and between populations obtained from microsatellite markers was greater than that of RAPD, indicating that microsatellite markers showed higher individual polymorphism than RAPD markers Sex.