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了解细胞调亡和Bcl－2蛋白表达在慢性胃炎、胃溃疡、胃粘膜不典型增生、胃癌中的规律和可能作用。方法：采用原位末端标记法（TUNEL法）检测细胞凋亡，采用免疫级化检测Bcl－2蛋白的表达。结果：慢性活动性胃炎、胃溃疡、轻度不典型增生、重度不典型增生、早期胃癌和进展期胃癌的凋亡指数分别为16．8±12．3％、24．1±20．0％、19．3±16．4％、15．7±15．2％、10．1±9．1％和6．3±6．0％，进展期胃癌显著低于早期胃癌和不典型增生（P＜0．05）。而Bcl－2蛋白阳性率分别为9．4％、27％．6％、52．9％、75．0％、83．3％和46．7％，早期胃癌显著高于进展期胃癌。在Lauren分型中，杨型和弥散型胃癌的细胞凋亡指数、Bcl－2蛋白的表达分别为8．3±7．2％、38．9％和5．1±4．9％、58．3％，两型比较，细胞调亡指数及Bcl－2蛋白表达均有显著差异（P＜0．05）。结论：在胃粘膜不典型增生、早期胃癌到进展期胃癌的发展中，细胞凋亡逐渐受到抑制。胃癌的形成与Bcl－2的高表达有关，而且主要的作用阶段可能在癌变早期。胃癌的Lauren分型与细胞凋亡指数及BcL－2蛋白表达密切相关。
To understand the regularity and possible role of apoptosis and Bcl-2 protein expression in chronic gastritis, gastric ulcer, gastric dysplasia, and gastric cancer. Methods: Apoptosis was detected by TUNEL method. The expression of Bcl-2 protein was detected by immunohistochemistry. Results: The apoptotic indexes of chronic active gastritis, gastric ulcer, mild dysplasia, severe dysplasia, early gastric cancer and advanced gastric cancer were 16.8±12.3% and 24.1±20.0%, respectively. , 19.3±16.4%, 15.7±15.2%, 10.1±9.1%, and 6.3±6.0%, significantly lower in advanced gastric cancer than early gastric cancer and dysplasia ( P<0.05). The positive rate of Bcl-2 protein was 9.4% and 27%, respectively. 6%, 52.9%, 75.0%, 83.3% and 46.7%. Early gastric cancer was significantly higher than advanced gastric cancer. In the Lauren classification, the apoptotic index and Bcl-2 protein expression were 8.3±7.2%, 38.9%, and 5.1±4.9%, respectively, in popliteal and diffuse gastric cancers. Compared with the two types, the apoptosis index and Bcl-2 protein expression were significantly different (P<0.05). Conclusion: In the development of gastric dysplasia, early gastric cancer and advanced gastric cancer, apoptosis is gradually inhibited. The formation of gastric cancer is related to the high expression of Bcl-2, and the main stage of action may be early in cancer. The Lauren classification of gastric cancer is closely related to the apoptosis index and the expression of BcL-2 protein.