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目前认为胰岛素依赖型糖尿病(IDDM)系自身免疫病,其发病的始动因子为病毒感染,发病机理中涉及细胞免疫异常。我们用单克隆抗体技术测定了16例病程在一年半以上的IDDM者胰岛移植前后的T淋巴细胞亚群,并与18名健康人进行对照。 (1)对象:18名健康人,男10人,女8人;平均年龄24.5±6.86岁,IDDM16例,男9例,女7例,平均年龄28.32±6.12岁,病程1.5～8年。全部受检者都抽血查T淋巴细胞亚群,且IDDM者在胰岛移植后15天(9人)、30天(7人)再复查T淋巴细胞亚群。(2)T淋巴细胞亚群检测:用上海试剂厂生产的淋巴细胞分离液分离外周血单个核细胞。用兔抗BALB/C小鼠γ球蛋白抗体致敏葡萄球菌A蛋白(SPA)菌体。
Insulin-dependent diabetes mellitus (IDDM) is currently considered Department of autoimmune disease, the onset of the initiating factor for viral infection, the pathogenesis involved in cellular immune disorders. We measured the T lymphocyte subsets of 16 IDDM patients before and after islet transplantation with a duration of more than one and a half years by monoclonal antibody technique and compared them with 18 healthy people. (1) Subjects: 18 healthy people, 10 males and 8 females. The average age was 24.5 ± 6.86 years. There were 16 cases of IDDM, 9 males and 7 females, with an average age of 28.32 ± 6.12 years and a duration of 1.5 to 8 years. T lymphocyte subsets were drawn from all subjects, and TDM subsets were retested in IDDM patients 15 days after transplantation (9) and 30 days (7). (2) Detection of T lymphocyte subsets: Peripheral blood mononuclear cells were isolated using lymphocyte separation liquid produced by Shanghai Reagent Factory. Staphylococcal protein A (SPA) bacteria were sensitized with rabbit anti-BALB / C mouse gamma globulin antibody.