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目的探索辐射诱发细胞恶性转化相关基因。方法本实验利用３’端引物ＨＴ１１Ｍ（Ｇ，Ｃ，Ａ）与５’端引物ＨＡＰ１７～２４所配的２４对引物同时对ＳＨＴＦ（ＳＶ４０ｉｍｍｏｒｔａｌｉｚｅｄｈｕｍａｎｆｅｔａｌｔｒａｃｈｅａｌｆｉｂｒｏｂｌａｓｔＳＨＴＦ）细胞和经２３８Ｐｕα粒子照射后能在软琼脂上形成克隆的此细胞（αＳＨＴＦ）的ｍＲＮＡ进行反转录ＰＣＲ扩增，６％测序胶电泳，放射自显影。结果发现有２３条基因片段在αＳＨＴＦ细胞中表达，ＳＨＴＦ细胞中不表达。回收差异的基因片段，随机取其中１２条重新扩增后得到６条扩增片段。其中Ｃ１７－５，Ｃ２３－１两片段经Ｎｏｒｔｈｅｒｎｄｏｔ杂交后，均与αＳＨＴＦ细胞ＲＮＡ有强阳性杂交信号，与ＳＨＴＦ细胞ＲＮＡ无杂交信号。结论本实验证实了ＳＨＴＦ和αＳＨＴＦ细胞的基因表达有差异存在。
Objective To explore the radiation-induced malignant transformation of genes. Methods In this experiment, SHTF (SV40immortalized human lefaltrafibroblast SHTF) cells and 238Puα particles were used to form clones on soft agar, using 24 pairs of primers matched with 3 ’end primer HT11M (G, C, A) and 5’ end primer HAP17-24 The mRNA of this cell (αSHTF) was subjected to reverse transcription PCR amplification, 6% gel electrophoresis and autoradiography. As a result, 23 gene fragments were found in αSHTF cells, but not in SHTF cells. Recover the difference of the gene fragments, which randomly selected 12 re-amplified 6 amplified fragments. The C17-5, C23-1 two fragments by Northerndot hybridization, with αSHTF cell RNA strong positive hybridization signal, and SHTF cell RNA no hybridization signal. Conclusion This experiment confirmed that the gene expression of SHTF and αSHTF cells are different.