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采用VIS和 ICP及酶活性测定等方法,研究铜锌超氧歧化酶(CU2ZN2SOD)与组氨酸钴(Ⅱ)［CO(HIS)N］ 的直接相互作用及外加组氨酸钴(Ⅱ)、磷酸盐对此类相互作用的影响.结果发现,水溶液中原酶活性中心的CU(Ⅱ)和ZN(Ⅱ)离子可被外加的组氨酸钴(Ⅱ)部分诱导、交换出来,而CO(HIS)N 中的CO(Ⅱ)进入酶的活性中心,形成“CO－SOD”酶衍生物各组分,并相应影响了酶的催化活性.与此同时,外加组氨酸钴(Ⅱ)及磷酸盐对此类相互作用有着重要的影响“,”The effect of changing the amount of external added Co(His ) n and phosphate on the interaction of Cu 2Zn 2SOD with organic metal compound (Cobalt(Ⅱ) Histidine) have been studied by means of ICP,VIS and measurement of enzyme activity.It has found that in aqueous solution,there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu 2Zn 2SOD) with external added Co(His ) n.As a result,part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives (Co(Ⅱ) substituted derivatives of SOD) were produced and the catalytic activity of enzyme were affected.It has also studied the intensity of interactions in different molar ratios (Cobalt(Ⅱ) Histidine) and of the presence of phosphate and got some results under the effect of corresponding factors.