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研究了在氧化镍纳米粒子改性石墨电极(GE)上肌红蛋白(Mb)的直接电化学行为,并制备了新型H2O2传感器。在0.1mol/L磷酸盐缓冲溶液(PBS,pH7.0)中,肌红蛋白有稳定而明确的氧化还原峰,电子转移速率常数为6.48/s;式量电位为-0.34V(vs·SCE),表面覆盖量8.06×10-10mol/cm2。二甲亚砜(DMSO)的存在对加速肌红蛋白分子与电极之间的电子传递起了重要作用。光谱分析表明:固定在Mb/NiO/DMSO膜中肌红蛋白能保持其生物活性,对H2O2有电催化活性,电催化响应与H2O2浓度呈线性关系,线性范围为0.8～24μmol/L;检出限为0.039μmol/L。对H2O2的表观米氏常数为0.21mmol/L,灵敏度为417mA cm2L/mol,呈现出高亲和性。
The direct electrochemical behavior of myoglobin (Mb) on nickel oxide nanoparticle modified graphite electrode (GE) was studied and a novel H2O2 sensor was prepared. In 0.1mol / L phosphate buffer solution (PBS, pH7.0), myoglobin has a stable and well-defined redox peaks, the electron transfer rate constant is 6.48 / s; the mass potential is -0.34V (vs · SCE ), The surface coverage of 8.06 × 10-10mol / cm2. The presence of dimethyl sulfoxide (DMSO) plays an important role in accelerating the electron transport between the myoglobin molecule and the electrode. The results of spectroscopic analysis showed that myoglobin retained its biological activity in Mb / NiO / DMSO membrane and had electrocatalytic activity on H2O2. The linear relationship was found between the electrocatalytic response and the concentration of H2O2. The linear range was 0.8 ~ 24μmol / L. Limit is 0.039 μmol / L. The apparent Michaelis constant for H2O2 was 0.21 mmol / L with a sensitivity of 417 mA cm2 L / mol, exhibiting high affinity.