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Liangyoupeijiu is a super high-yield hybrid rice.Despite its advantages with respect to yield and grain quality,it is sensitive to cold,which keeps it from being widely cultivated.We subjected Liangyoupeijiu seedlings to 4℃cold treatment,then extracted the leaf proteins.After 2-D gel electrophoresis separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis,a series of differentially displayed proteins were identified.Some metabolism-associated proteins were found among the downregulated proteins,such as carbamoyl phosphate synthetase,transketolase 1,dihydrolipoamide dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase.The upregulated proteins included both stress-resistance proteins such as nucleoside diphosphate kinaseⅠand proteins that are negative for rice growth,such as FtsH-like protein,plastid fusion and/or translocation factor(Pftf) and actin.Our results indicate that cold may inhibit Liangyoupeijiu growth through decreasing metabolic activity and damaging cell structure.
Liangyoupeijiu is a super high-yield hybrid rice. Despite its advantages with respect to yield and grain quality, it is sensitive to cold, which keeps it from being provided substantially cultivated. We Lian Liangyoupeijiu seedlings to 4 ℃ cold treatment, then extracted the leaf proteins . After 2-D gel electrophoresis separation and matrix-assisted laser desorption / ionization time-of-flight mass spectrometry analysis, a series of differentially displayed proteins identified. Home metabolism-associated proteins were found among the downregulated proteins, such as carbamoyl phosphate synthetase, transketolase 1, dihydrolipoamide dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase. The upregulated proteins are both stress-resistance proteins such as nucleoside diphosphate kinase I and proteins that are negative for rice growth, such as FtsH-like protein, plastid fusion and / or translocation factor (Pftf) and actin.Our results indicate that cold may inhibit Liangyoupeijiu growth through decreasing m etabolic activity and damaging cell structure.