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目的建立X射线照射离体人血诱导染色体畸变的剂量-效应曲线。方法采用健康男性肘静脉血,经照射剂量为0.00~5.00 Gy、剂量率为0.8 mGy/s的X射线离体照射,以培养开始加秋水仙素法培养细胞,常规制片,经吉姆萨染色后,采用盲法阅片,记录中期细胞的染色体双着丝粒体、多着丝粒体和双着丝粒环及其伴随断片(以下简称“双+环”),拟合“双+环”率与照射剂量的剂量-效应曲线。结果在0.00~5.00 Gy范围内“双+环”率和畸变细胞率均随照射剂量增加而增高(P<0.01)。0.00~1.00 Gy的最佳拟合方程为y=23.22 D2+4.768 D-0.018(P<0.01);0.50~5.00 Gy的最佳拟合方程为y=34.23 D-3.072(P<0.01)。结论所建立的剂量-效应曲线拟合度较好,可为本实验室评估照射量提供参考依据。
Objective To establish a dose-response curve of chromosome aberration induced by X-ray irradiation in vitro. Methods The healthy male elbow venous blood was irradiated by X-rays at a dose of 0.00-5.000 Gy and a dose rate of 0.8 mGy / s. The cultured cells were cultured with colchicine, and the cells were routinely prepared. After Giemsa staining After the blind reading, the cells of the metaphase cells were recorded, the mitochondria, the mitochondria and the dicentric rings and their associated fragments (hereinafter referred to as “double + ring” Double + ring “dose rate and dose-effect dose curve. Results In the range of 0.00-5.00 Gy, the ”double loop" rate and the rate of aberrant cells increased with the increase of irradiation dosage (P <0.01). The best fit equation of 0.00 ~ 1.00 Gy was y = 23.22 D2 + 4.768 D-0.018 (P <0.01). The best fit equation of 0.50-5.00 Gy was y = 34.23 D-3.072 (P <0.01). Conclusion The fitted dose-response curve is good, which can provide a reference for the laboratory to evaluate the irradiation dose.