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以黄瓜(Cucumis sativus L.)种子为试材,研究了外源H2S预处理对镉胁迫下黄瓜胚轴和胚根生理生化特性的影响。结果表明:用浓度为300、600、900和1200μmol·L-1的H2S供体NaHS预处理黄瓜种子均能显著缓解镉胁迫对其胚轴和胚根的抑制作用,其中以900μmol·L-1NaHS处理效果最好。NaHS处理显著提高了镉胁迫下黄瓜子叶中淀粉酶的活性及胚轴与胚根中SOD、POD、CAT和APX活性,提高了二苯代苦味酰基自由基(DPPH·)清除能力和羟基自由基(OH·)清除能力,从而降低了MDA和H2O2含量,而其他钠盐(Na2S、Na2SO4、NaHSO4、Na2SO3、NaHSO3和NaAc)处理效果甚微,表明NaHS缓解镉胁迫对黄瓜胚轴和胚根生长的抑制作用归因于其释放出的H2S。
Cucumber (Cucumis sativus L.) seeds were used as experimental materials to study the effects of exogenous H2S pretreatment on physiological and biochemical characteristics of cucumber hypocotyl and radicle under cadmium stress. The results showed that pretreatment of cucumber seeds with H2S donor NaHS at concentrations of 300, 600, 900 and 1200 μmol·L-1 significantly alleviated the inhibitory effect of cadmium stress on the hypocotyls and radicles, with 900 μmol·L-1 NaHS The best treatment. NaHS treatment significantly increased amylase activity and activities of SOD, POD, CAT and APX in cucumber cotyledons under cadmium stress and enhanced scavenging ability of DPPH · and hydroxyl radical (OH ·) scavenging ability, which reduced the contents of MDA and H2O2. However, the effects of other sodium salts (Na2S, Na2SO4, NaHSO4, Na2SO3, NaHSO3 and NaAc) The inhibition is due to the release of H2S.